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Counting Platelets
COUNTING PLATELETS: A REVIEW OF AUTOMATED TECHNOLOGIES

Automated hematology analyzers use different approaches to accurately resolve Platelet (PLT) populations from other cellular elements of the blood. These options include electrical impedance, optical flow cytometry and fluorescent methods. The reference method for PLT counts is dual-color flow cytometry (CD41/61), but it is resource-intensive and expensive, and is not practical for use in routine laboratory practice.

Technologies that are routinely used for PLT enumeration have different performance characteristics and sensitivities to common interferences, especially at the low end of the PLT measuring range. These interfering factors include low volume red blood cells (RBCs), like microcytes, schistocytes and spherocytes, fragmented white blood cells (WBCs), cryoglobulins, lipemia (hyperchylomicronemia and infusion with lipid emulsions) and the presence of giant PLTs and PLT clumps. Understanding the measurement principles and the associated performance characteristics can help guide decision making.
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